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Widefield and Confocal Microscopy

 

 

 

 

 

 

http://www.microscopyu.com/articles/formulas/formulasindex.html

http://micro.magnet.fsu.edu/primer/anatomy/anatomy.html

http://scienceinyoureyes.memphys.sdu.dk/konfokalmikroskopi_en.php

 

 

Confocal microscopy allows us to image many XY slices to form a reconstructed image of a vesicle.  With the z-dimensional accuracy, we examine the XZ and YZ planes for vesicle deformation on surfaces.  The example to the right shows the information available from a confocal image.  The two-phase vesicle on the right is a widefield image of a similar vesicle.

 

Surface Plasmon Resonance Imaging